Collagen is the matrix component of the dermis, which is mainly composed of type I collagen and type III collagen. Type I collagen is located in the deep dermis, with a large amount and thick fibers in a bundle shape, playing a supporting role; Type III collagen is mostly located in the superficial layer of the dermis, with a small amount, fine fibers, and a sparse network, making the skin elastic.

The main basic unit of collagen is the collagen tripeptide unit - glycine X-Y (where X includes proline or hydroxyproline, Y includes serine, glutamic acid, etc.). Soluble type I collagen has a molecular weight of less than 300000, and collagen has the characteristic of being easily soluble in acidic aqueous solutions.

Type I collagen raw materials include animal derived bovine bones, tendons, pig skin, fish skin, etc; Type I collagen has a wide molecular weight distribution range and a large yield, and its uses include medicinal excipients, medical devices, cosmetics, etc. The molecular weight of type III collagen is about 55KD, and the product mainly comes from recombinant bacterial fermentation. Due to its 100% human origin, it has the advantage of no allergic reactions and no rejection, and is mainly used in medical and cosmetics.

The production process of type I collagen includes deacidification and desalination of collagen solution, removal of small molecule derivatization reagents, removal of small molecule weight collagen and collagen peptides, concentration of collagen, etc. The production process of type III collagen mainly includes the clarification of recombinant bacterial crushing solution, the removal of high and low molecular weight impurities in collagen, and the exchange and concentration of collagen eluent. Product quality control indicators include: purity of main components, impurity content, pH, conductivity, clarity, concentration, etc.

Collagen has the characteristics of high molecular weight and high solution viscosity. Shandong Bona Biotechnology Group Co., Ltd. has fully achieved the process goals and product batch production of collagen by selecting appropriate filter membrane types and accuracy, combined with membrane filtration systems.

The membrane filtration system has many advantages in processing collagen raw materials:

1. Normal temperature, mild conditions, no component damage, especially suitable for heat sensitive substances;

2. Pure physical filtration, no phase change or qualitative change, without damaging the active ingredients;

3. High filtration accuracy, uniform pore size distribution, enabling the purification and concentration of effective ingredients;

4. The system is easy to operate, has a short process, and is easy to clean and maintain;

5. The material liquid treated by the membrane is clear and stable in nature, which is conducive to further processing in subsequent steps;

6. The system is designed for cross flow operation, without the need for additives or the introduction of new impurities, completely solving the problem of pollution and blockage;

7. Modular design of the system, convenient replacement of membrane components, design of online cleaning and sewage discharge devices, reducing labor intensity and production costs, and improving production efficiency;

8. Select stainless steel sanitary materials that comply with QS quality standards;

9. The system is automatically controlled by PLC, with low labor intensity. The membrane filtration process is carried out in a closed container, achieving clean production;

10. The membrane filtration element has a large filling area and a small system footprint, making it convenient for technical improvement, expansion, or new construction projects, effectively reducing investment and production costs.