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Solid phase extraction SPE

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Product Introduction Typical application areas Partial user units Bona Technology Advantages

Product Introduction

Solid Phase Extraction (SPE) is a sample pretreatment technology that has been developed since the mid-1980s. Developed by combining liquid-solid extraction and liquid chromatography technology. Mainly used for sample separation, purification, and enrichment. The main purpose is to reduce sample matrix interference and improve detection sensitivity. Compared with traditional sample pretreatment methods, solid-phase extraction has many advantages such as higher efficiency, better effectiveness, easy to achieve quantitative extraction, and fast and automated implementation; And the use of solid-phase extraction technology can remove complex sample matrix interference, improve sample purity and quality, and extend the service life of the chromatographic system and column.

The SPE products provided by Bona Bio have a complete range of fixed phases, excellent separation efficiency, high recovery rate, and good repeatability between batches. The product quality can be comparable to imported brand products such as WATERS.

 

The solid phase extraction column products that Bona Bio can provide are as follows: 

 

 

Model

product name

Product Features

specifications

application

SPE-MCX

Mixed-mode Cation Exchanger SPE Column

(Mixed cation solid-phase extraction column)

A mixed adsorbent with strong cation exchange and reverse retention effects. Highly selective for alkaline analytes, stable in organic solvents.

1cc/30mg

1cc/60mg,

3cc/60mg

3cc/100mg,

6cc/150mg

6cc/200mg,

6cc/500mg

Monitoring of alkaline drugs in biological fluids and tissue extracts includes screening, identification, confirmation, and quantitative analysis of insecticides and herbicides

SPE-WCX

Weak Cation Exchanger SPE Column

(Weak cation solid-phase extraction column)

The composite mechanism of weak cation exchange and reverse retention. Used to retain and release strongly alkaline analytes. Stable in organic solvents.


Drug monitoring of strong alkaline drugs in biological fluid and tissue extracts includes: screening, identification, confirmation and quantitative analysis of streptomycin and dihydro streptomycin in vegetables

SPE-MAX

Mixed-mode Anion Exchanger SPE Column

(Mixed anionic solid-phase extraction column)

A mixed adsorbent with strong anion exchange and reverse retention effects. Highly selective for acidic analytes, stable in organic solvents.

1cc/30mg

1cc/60mg,

3cc/60mg

3cc/100mg,

6cc/150mg

6cc/200mg,

6cc/500mg

Monitoring of acidic drugs in biological fluids and tissue extracts includes screening, identification, confirmation, and quantitative analysis of food additives and pollutants

SPE-WAX

Weak Anion Exchanger SPE Column

(Weak anion solid-phase extraction column)

The composite mechanism of weak anion exchange and reverse retention. Used to retain and release strongly acidic analytes. Stable in organic solvents.


Monitoring of strongly acidic drugs in biological fluids and tissue extracts includes screening, identification, confirmation, and quantitative analysis of food additives and pollutants

SPE-HLB

Hydrophilic–Lipophilic Balance SPE Column

Hydrophilic Ester Solid Phase Extraction Column

Hydrophilic lipophilic equilibrium chemical structure, strong hydrophobic water wettable polymer adsorbent, even when dry, still retains the analyte well. Stable in many organic solvents.

1cc/30mg

1cc/60mg,

3cc/60mg

6cc/150mg

6cc/200mg

6cc/500mg

Extraction and purification of drug metabolites in body fluids;

Separation of peptides and oligonucleotides High throughput biological macromolecule desalination Trace organic substances, endocrine disruptors such as antibiotics in food: fluoroquinolone, tulamycin, cephalosporin, chloramphenicol pesticide residues: sulfonylureas, herbicides, carbamates, pesticides, sulfonamides and β- Lactam antibiotics: diazepam estrogen

Diethylstilbestrol tetracycline macrolide nitroimidazole acrylamide


SPE-RPC

ReversePhaseChromatography SPE Column

(Reverse phase solid-phase extraction column)

Polymer adsorbent based on styrene/divinylbenzene

1cc/30mg

1cc/60mg,

3cc/60mg

3cc/100mg

6cc/200mg

6cc/500mg,

Used for rapid adsorption and separation of hydrophobic substances such as phenol surfactants, bromoethylpyridine amino acids and peptide compounds

SPE-SIL

Silica SPE Column

(Silicone solid-phase extraction column)

Polar adsorbents are mainly used to adsorb analytes from non-polar solvents such as hydrocarbon haloalkane or ester or ether with weaker polarity, and then elute with more polar solvents such as polar ether ester alcohol acetonitrile water. It can also be used as a medium strength cation exchange adsorbent in aqueous media, and as a carrier for liquid-liquid distribution and separation.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg, 6cc/1000mg

Vitamin food additives

Organic compounds for lipid synthesis

Insecticides in natural product plant pigment foods


SPE-C18

C18-Reverse-Phase SPE Column

C18 reverse phase solid-phase extraction column)

Hydrophobic silica gel matrix C18 bonded phase is used for enrichment and extraction of analytes in aqueous samples.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg

6cc/1000mg

12cc/2000mg

Segmented collection of lipids: isolation of gangliosides

Natural products of organic acids in fruit juice wine

Analysis of pigments and sugars in food


SPE-CN

CN SPE Column

(Cyanide solid phase extraction column)

The weakly hydrophobic silicone matrix cyanopropyl bonded phase can serve as an adsorbent with weaker polarity than silica gel under normal phase conditions; Alternatively, it can be used as an adsorbent with weaker hydrophobicity than C18/C8 under reverse phase conditions.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg, 6cc/1000mg

Drugs and their metabolites

pesticide


SPE-NH2

NH2 SPE Column

(Amino solid-phase extraction column)

Silicone matrix with moderate polarity and aminopropyl bonding phase, with weak alkalinity on the surface of the adsorbent. It can serve as a polar adsorbent with different selectivity for acidic/alkaline analytes. It can also serve as a weak anion exchanger in water substrates below pH 8.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg, 6cc/1000mg

Phenol, phenolic pigments, natural products

Petroleum distillate

sugar

Drugs and their metabolites


SPE-PSA

PSA SPE Column

Ethylenediamine-N-propyl solid-phase extraction column

The silica gel based primary/secondary amine bonded phase exhibits selectivity similar to the aminopropyl bonded phase, but its PKa value is higher and its ion exchange capacity is higher. PSA can also chelate with metal ions for the extraction of metal ions.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg, 6cc/1000mg

Strong affinity for fatty acids, polar pigments, and sugars

Possible use for adsorption under chelation mechanism


SPE-PRS

PRS SPE Column

(Propylene sulfonic acid solid-phase extraction column)

A strong cation exchange extraction column based on silica gel. The bonding functional group is propylsulfonic acid, with slightly lower acidity than benzenesulfonic acid. Used for extracting weak cations.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg, 6cc/1000mg

Pretreatment of Malachite Green Samples

SPE-AluN

Neutral-Alumina SPE Column

(Neutral alumina solid-phase extraction column)

Highly surfactant polar adsorbent, similar to silica gel, has three types: neutral (N) acidic (A) alkaline (B). There are specific π electron interactions with aromatic hydrocarbon analytes. Acid alkaline alumina can serve as a low capacity ion exchanger in aqueous matrices, unaffected by high energy activity, and is more stable than silica gel under extreme pH conditions.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg

6cc/1000mg

Synthesis of organic compounds from petroleum fractions as food/feed additives

Purification and Separation of Pesticide and Herbicide Contaminants


SPE-AluB

Basic-Alumina SPE Column

(Alkaline alumina solid-phase extraction column)



Purification and Separation of Pesticide and Herbicide Contaminants

Radioactive compound isotope

Phospholipid steroid catecholamines


SPE-AluA

Acid-Alumina SPE Column

(Acid alumina solid-phase extraction column)



Food/feed additives

Radioactive compound isotope


SPE-CEL

Celite SPE Column

(Diatomaceous earth solid-phase extraction column)

Diatomaceous earth treated with special processes has a large specific surface area and low surface activity, which can provide an ideal support surface for liquid-liquid distribution. Suitable for alternative liquid-liquid extraction methods.

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg

6cc/1000mg

Drug analysis in plasma samples

Analysis of active ingredients in complex samples


SPE-Flo

Florisil SPE Column

(Florida silica solid-phase extraction column)

Highly active and weakly alkaline adsorbent with high polarity, used to adsorb low to medium polarity analytes from non aqueous solutions

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg

6cc/1000mg

insecticide

Insecticides in food

Polychlorinated biphenyls in transformer oil


SPE-CAR

Carbon SPE Column

(Graphitized carbon black solid phase extraction column)

Used for polar analyte extraction, the adsorption capacity of compounds is more than twice that of silica gel. The regular six membered ring structure provides strong affinity for planar molecules with minimal background interference

1cc/100mg,

3cc/200mg

3cc/500mg,

6cc/500mg

6cc/1000mg

Extraction and purification of organic matter

Separation and removal of various substrates such as pigments, sterols, phenols, organochlorine pesticides, etc. in fruits and vegetables


SPE-C/N

Carbon/NH2  SPE Column

Graphite carbon black/amino composite solid-phase extraction column

Double layer column bed adsorbent for sample purification before analyzing multiple pesticide residues in food

6cc/300mg/500mg

6cc/500mg/500mg

Analysis of pesticide residues in food

Removal of pigments, fatty acids, and phenols

Extraction of Organic Phosphorus with Aniline



Typical application areas

Partial user units

部分使用单位

Bona Technology Advantages

Complete multiple membrane equipment

Engineers with membrane engineering

Professional technical support provided


A comprehensive customer service system

Provide high-quality maintenance services

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